Receptor proteins for concanavalin A and wheat germ agglutinin of bovine milk fat globule membrane probed by affinity chromatography in the presence of sodium dodecyl sulfate.
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چکیده
منابع مشابه
Bovine milk fat globule membrane proteome.
Milk fat globule membranes (MFGM) were isolated from the milk of mid-lactation Holstein cows. The purified MFGM were fractionated using 1-dimensional SDS gels. Tryptic peptides from gel slices were further fractionated on a micro-capillary high performance liquid chromatograph connected to a nanospray-tandem mass spectrometer. Analysis of the data resulted in 120 proteins being identified by tw...
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this study investigates the cohesive devices used in the textbook of english for the students of psychology. the research questions and hypotheses in the present study are based on what frequency and distribution of grammatical and lexical cohesive devices are. then, to answer the questions all grammatical and lexical cohesive devices in reading comprehension passages from 6 units of 21units th...
Interaction of concanavalin A and wheat germ agglutinin with the insulin receptor of fat cells and liver.
Wheat germ agglutinin enhances the specific binding of insulin to isolated fat cells and to liver cell membranes at a concentration of about 1 pg per ml. Wheat germ agglutinin increases insulin binding by increasing the rate of insulinreceptor complex formation; the protein does not alter the rate of dissociation of the insulin-membrane complex or the total number of binding sites for insulin. ...
متن کاملMolecular-weight estimates of milk-fat-globule-membrane protein-sodium dodecyl sulphate complexes by electrophoresis in gradient acrylamide gels.
The molecular weights of milk-fat-globule-membrane proteins solubilized in sodium dodecyl sulphate were estimated by gradient gel electrophoresis. Standard curves were calibrated from both protein and glycoprotein markers of known molecular weight. Six major proteins were observed with Coomassie Blue staining and six with periodic acid-Schiff staining. The behaviour of the membrane proteins and...
متن کاملPreparation of monoclonal antibodies to xanthine oxidase and other proteins of bovine milk-fat-globule membrane.
Nine hybridomas secreting monoclonal antibody to proteins of bovine milk-fat-globule membrane were isolated. All nine cell lines continued to secrete monoclonal antibody after serial transfer in culture and after passage as solid tumours in Balb/cJ mice. Four of the cell lines secreted monoclonal antibody specific for xanthine oxidase, one of the major proteins of milk-fat-globule membrane.
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1986
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.50.2997